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Efficient Navigation (Zen) Black Edition Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl <t>Zeiss;</t> <t>ZEN-software).</t> For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).
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Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl Zeiss; ZEN-software). For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

doi: 10.3389/fbioe.2020.01016

Figure Lengend Snippet: Dynamic Ca 2+ mobilization in vital fluo-3 loaded MG-63s growing on amino polymers, extracellular matrix (ECM)/peptide motifs and polyelectrolyte multilayers (PEM) in the time frame 0–480 s. The positive control PPAAm (also an amino polymer) and the negative control Ti are embedded for the respective experiment. Shown are time courses of the mean fluorescence intensity of cells (MFI C ) over 8 min: First, the basal Ca 2+ signal is recorded for 180 s. At 180 s the cells are stimulated with ATP (arrowheads) and the Ca 2+ signal is detected (LSM780, Carl Zeiss; ZEN-software). For statistical analyses of the basal MFI, values of the time points 0–170 s are used. For analyses of the MFI after ATP stimulation, values of the time points 190–240 s are used (highlighted in grey). MFI C values are normalized to the mean cell area after 24 h. (Statistics: Kruskal–Wallis and Dunn’s multiple comparison test, p < 0.05; mean ± sem; n ≥ 3 independent approaches, per time point ≥ 30 cells; * indicates significance of modification x to Ti and # significance to PPAAm).

Article Snippet: Oil DIC M27) and the ZEN software (ZEISS efficient navigation, ZEN 2011 SP4, black edition, Carl Zeiss).

Techniques: Positive Control, Polymer, Negative Control, Fluorescence, Software, Comparison, Modification